rabbit polyclonal anti-pad2  (Cosmo Bio USA)

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Reciprocal regulation of protein arginine deiminase 2 and 4 expression in the colonic mucosa of ulcerative colitis

doi: 10.3164/jcbn.23-77

Figure Lengend Snippet: mRNA expression of protein arginine deiminase 2 and 4 in patients with inflammatory bowel diseases. mRNA was isolated from the ileal and colonic mucosa of patients with ulcerative colitis (UC; active disease, n = 20; remitted disease, n = 20) and Crohn’s disease (CD; active disease, n = 10; remitted disease, n = 10). Non-tumorous portions of the colonic mucosa in patients with colon adenoma served as healthy colonic mucosa (HC, n = 4). qRT-PCR analysis of the mRNA expression levels of protein arginine deiminase (PAD)2 and PAD4. Each dot represents the value for each patient. (A) Data are presented as the mean ± SE. ** p <0.01, N.S.; not significant. (B) Correlation between PAD2 and PAD4 mRNA expression in patients with UC and CD. P values and correlation coefficient ( r ) values, as determined by Pearson’s correlation coefficient, are shown.

Article Snippet: PAD2 and PAD4 expression was visualized using the DAKO EnVision+ System (DAKO JAPAN, Tokyo, Japan), as previously described, with rabbit human anti-PAD2 antibody (Proteintech, Rosemont, IL) and anti-PAD4 antibody (GeneTex, Irvine, CA). ( )

Techniques: Expressing, Isolation, Quantitative RT-PCR

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Reciprocal regulation of protein arginine deiminase 2 and 4 expression in the colonic mucosa of ulcerative colitis

doi: 10.3164/jcbn.23-77

Figure Lengend Snippet: Correlation between the mRNA expression of protein arginine deiminase 4 ( PAD4 ) and cytokines in patients with ulcerative colitis (UC). mRNA was isolated from the colonic mucosa of patients with UC. (A) Correlation between the mRNA expression of PAD4 and IFN-α4 , IFN-β , IL12/23p40 , C-X-C motif chemokine ligand 8 ( CXCL8 ), CXCL10 , TNF receptor-associated factor 3 ( TRAF3 ), interferon regulatory factor 3 ( IRF3 ), IRF7 , IL-6 , and TNF -α. (B) Correlation between the mRNA expression of PAD2 and IFN-α4 , IFN-β , CXCL8 , CXCL10 , IL-6 , and TNF -α. Each dot represents the value for each patient. P values and correlation coefficient ( r ) values, as determined by Pearson’s correlation coefficient, are shown.

Article Snippet: PAD2 and PAD4 expression was visualized using the DAKO EnVision+ System (DAKO JAPAN, Tokyo, Japan), as previously described, with rabbit human anti-PAD2 antibody (Proteintech, Rosemont, IL) and anti-PAD4 antibody (GeneTex, Irvine, CA). ( )

Techniques: Expressing, Isolation

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Reciprocal regulation of protein arginine deiminase 2 and 4 expression in the colonic mucosa of ulcerative colitis

doi: 10.3164/jcbn.23-77

Figure Lengend Snippet: Correlation between the mRNA expression of protein arginine deiminase 4 ( PAD4 ) and cytokines in patients with Crohn’s disease (CD). mRNA was isolated from the ileal and colonic mucosa of patients with CD. (A) Correlation between the mRNA expression of PAD4 and IFN-α4 , IFN-β , IL12/23p40 , C-X-C motif chemokine ligand 8 ( CXCL8 ), CXCL10 , TNF receptor-associated factor 3 ( TRAF3 ), interferon regulatory factor 3 ( IRF3 ), IRF7 , IL-6 , and TNF-α . (B) Correlation between the mRNA expression of PAD2 and IFN-α4 , IFN-β , CXCL8 , CXCL10 , IL-6 , and TNF-α . Each dot represents the value for each patient. P values and correlation coefficient ( r ) values, as determined by Pearson’s correlation coefficient, are shown.

Article Snippet: PAD2 and PAD4 expression was visualized using the DAKO EnVision+ System (DAKO JAPAN, Tokyo, Japan), as previously described, with rabbit human anti-PAD2 antibody (Proteintech, Rosemont, IL) and anti-PAD4 antibody (GeneTex, Irvine, CA). ( )

Techniques: Expressing, Isolation